In the game, two Sailor Senshi fight each other using both basic physical and special attacks. Each Senshi has their own set of attacks, some of which are invented exclusively for the game. All of the Solar System Senshi, with the exception of Sailor Saturn, are playable characters.
There are four modes in the game: practice, tournament, versus and story mode. In each mode, the basic gameplay is the same. In versus and story mode, the player can customize the abilities of the chosen Senshi by distributing points among six ability scores.
- Practice Mode - In Practice mode, the players can practice their attacks with no counted damage.
- Tournament Mode - Eight players fight in a knock-out tournament, with 1-8 players being human and the others being CPU characters.
- Versus Mode - The player can fight one duel against another player or against a CPU player. The player chooses which Senshi should fight each other. It is possible to match a Senshi against herself; in this case, one of their sailor fukus would be altered.
- Story Mode - In Story Mode, the plot involved the Sailor Senshi fighting to choose a new leader. In this mode, only the Inner Senshi and Sailor Chibi Moon are playable characters. The player has to defeat the other eight normally playable Senshi to win in this mode. If the player succeeds, the title screen changes to display the silhouette and the name of the victorious Senshi. Tuxedo Mask appears in the "Continue" screen if the player looses a duel.
There are ten different stages in the game, including one hidden stage. They each have a different background, although the backgrounds do not affect the gameplay. and Each stage has a different inhabitant.
- Moon Castle - Sailor Moon
- Water Fountain at the Park ~ Day - Sailor Mercury
- Water Fountain at the Park ~ Night - Sailor Uranus
- Seaside Port - Sailor Neptune
- Crystal Tokyo ~ Day - Sailor Chibi Moon
- Crystal Tokyo ~ Night - Sailor Venus
- Hidden Stage - The hidden stage takes place inside an office building with various workers cheering on the fight.